Analysis Note

ControlRecombinant BDCA-2 protein

Application

Research Sub CategoryImmunoglobulins & Immunology

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BDCA-2 in human tonsil tissue.

Research CategoryInflammation & Immunology

Detect BDCA-2 using this Anti-BDCA-2 Antibody, clone 10E6.1 validated for use in Western Blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Blood dendritic cell antigen 2 (BDCA-2;CD303) is a type II C-type lectin that is expressed on plasmacytoid dendritic cells (PDC), a subset of immature dendritic cells found in lymphoid and nonlymphoid tissues. BDCA-2 is critically involved in both adaptive and innate immune responses. It is involved in the recognition and internalization of pathogens, but inhibits TLR6 signaling and INF-1 production, in PDC cells, possibly through signaling of the BCR-like signalosome. PDC signaling has been linked to multiple inflammatory diseases such as granulomatous lymphadenitis.

Immunogen

GST-tagged recombinant protein corresponding to human BDCA-2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in recombinant BDCA-2 protein.

Western Blot Analysis: 1 µg/mL of this antibody detected BDCA-2 on 10 µg of recombinant BDCA-2 protein.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~33 kDa observed. Uncharacterized bands appear at ~25 and ~31 kDa in some lysates.